Adipose tissue metabolism and cardiac function in obesity
Our main goal is (i) to examine the mechanisms by which dietary Calanus oil reduces fat deposition during high-fat feeding and (ii) whether the reduction in body fat has any impact of post-ischemic recovery of cardiac function in male mice.
Obesity is induced by feeding normal mice a high-fat diet (HFD) over a 12 wk period, followed by an 8 wk feeding period during which time the animals will be treated with a novel marine oil (Calanus oil). Mice fed normal chow is used as lean controls. Two days prior to termination of the experiment an intraperitoneal injection of a bolus of 2H2O in saline (3.5 ml per 100 g body weight) is given, and 5% of the drinking water is replaced by 2H2O. At the end of the experiment adipose tissue is dissected followed by extraction of lipids and separation of tri-acyl glycerol (TAG). Rates of TAG synthesis and de novo lipogenesis are assessed by in vivo 2H enrichment of TAG by nuclear magnetic resonance spectroscopy.
Hearts will be excised and perfused ex vivo for metabolic and functional measurements. Furthermore, blood and tissue samples are taken for analysis of metabolic parameters and gene expression.
Expected effects on the animals: based on previous experiments, dietary Calanus oil will cause slightly less fat deposition during high-fat feeding and improved insulin sensitivity compared to non-treated HFD mice
Expected scientific and or social utility value: Obesity is associated with insulin resistance and cardiovascular disease. Hence, interventions which can prevent the development of obesity (and reveal the biological mechanisms involved) will be important to combat these conditions.
Number of animals:48
The feeding/treatment schedule follows a strict plan, which allows maximum utilization of the animals.
Obesity is induced by feeding normal mice a high-fat diet (HFD) over a 12 wk period, followed by an 8 wk feeding period during which time the animals will be treated with a novel marine oil (Calanus oil). Mice fed normal chow is used as lean controls. Two days prior to termination of the experiment an intraperitoneal injection of a bolus of 2H2O in saline (3.5 ml per 100 g body weight) is given, and 5% of the drinking water is replaced by 2H2O. At the end of the experiment adipose tissue is dissected followed by extraction of lipids and separation of tri-acyl glycerol (TAG). Rates of TAG synthesis and de novo lipogenesis are assessed by in vivo 2H enrichment of TAG by nuclear magnetic resonance spectroscopy.
Hearts will be excised and perfused ex vivo for metabolic and functional measurements. Furthermore, blood and tissue samples are taken for analysis of metabolic parameters and gene expression.
Expected effects on the animals: based on previous experiments, dietary Calanus oil will cause slightly less fat deposition during high-fat feeding and improved insulin sensitivity compared to non-treated HFD mice
Expected scientific and or social utility value: Obesity is associated with insulin resistance and cardiovascular disease. Hence, interventions which can prevent the development of obesity (and reveal the biological mechanisms involved) will be important to combat these conditions.
Number of animals:48
The feeding/treatment schedule follows a strict plan, which allows maximum utilization of the animals.