Breeding mutant mouse strains
Mutant mouse strains are essential tools for dissecting the mechanisms involved in the development, activation, regulation and maintenance of the immune system. The mutant strains in this application will be used for various immunological studies. The mutants are either acquired from commercial vendors or generated in house. The mutants include transgenics, knock-ins, knock-outs and congenics. All mutants are on the BALB/c or C57BL/6 inbred mouse background strains. Relevant control strains will also be bred.
No experimental in vivo procedures will be performed other than those which are necessary for geno- or phenotyping individual animals which can involve the collection of ear tissue or blood. When possible we will phenotype by employing optical imaging using the IVIS Spectrum. All breeding will be guided by the ideals of sound animal husbandry practice. When ready for use, animals will either be transferred to other approved FOTS applications or used by harvesting organs from euthanized mice during or after terminal anesthesia.
This FOTS application will involve 30 different mutants, many are single mutants but some single mutants will be interbred to produce multiple mutants for specific applications. At least 10 other FOTS applications will be the receivers of offspring from this application. Due to the number of strains involved and the number of FOTS applications which will receive mice, we believe we will require a total of 4800 mice.
An advantage with a single breeding project with regards to the 3 Rs is the avoidance of breeding separately the same strain in several other FOTS applications.This will reduce the number of mice needed for the maintainance of each strain and also make better use of the offspring produced from a colony.
Five participants will gain access to the mouse strains in this FOTS application.
No experimental in vivo procedures will be performed other than those which are necessary for geno- or phenotyping individual animals which can involve the collection of ear tissue or blood. When possible we will phenotype by employing optical imaging using the IVIS Spectrum. All breeding will be guided by the ideals of sound animal husbandry practice. When ready for use, animals will either be transferred to other approved FOTS applications or used by harvesting organs from euthanized mice during or after terminal anesthesia.
This FOTS application will involve 30 different mutants, many are single mutants but some single mutants will be interbred to produce multiple mutants for specific applications. At least 10 other FOTS applications will be the receivers of offspring from this application. Due to the number of strains involved and the number of FOTS applications which will receive mice, we believe we will require a total of 4800 mice.
An advantage with a single breeding project with regards to the 3 Rs is the avoidance of breeding separately the same strain in several other FOTS applications.This will reduce the number of mice needed for the maintainance of each strain and also make better use of the offspring produced from a colony.
Five participants will gain access to the mouse strains in this FOTS application.