Establishment and passaging of xenografts in nude mice
1 Purpose:
The mice applied for in this application will be used to generate new PDX models from patient tissue, re-establish models from human cell lines/cryoperserved tissue and to maintain the growth of existing xenograft models. The tissue generated will be used to support the department of Tumor biology ex vivo and in vivo activities.
2 Impact on animal welfare:
The animals used in this application will be subjected to various engraftment procedures and be handled/restrained on a weekly basis (check ups). The tumor tissue grafted into the mice will be allowed to grow to endpoint before it is passaged into new mice. Animals that are getting close to end point might experience weight loss and deteriorating health. Depending on the growth rate of the xenograft the life expectancy for the animals after engraftment will vary from 30-90 days.
3 Benefit:
Patient-derived xenografts (PDX) are models of cancer where the tissue or cells from a patient's tumor are implanted into an immune deficient or humanized mouse. PDX models simulate human tumor biology allowing for natural cancer progression, and offer the most translational research model for evaluating efficacy.
4 Number of animals:
In this application we plan to use up to 2400 Athymic nude mice over a period of four years.
5 The three Rs
Replacement:
- So far no alternatives has been discovered that can be a substitute for the complex functions of a living, breathing, whole-organ system with pulmonary and circulatory structures like those in humans. Until such a discovery, animals must continue to play a critical role in helping researchers understand the biology of cancer.
Reduction:
- The xenograft models that are not in use will be cryopreserved and stored untill needed.
- Passaging is done into a small number of animals, usually 2-3 animals per passage.
- Tissue generated for ex vivo experiments will be shared between researchers.
Refinement.
- Environmental enrichment will be provided in form of the addition of cardboard houses, plastic tubes, and/or shredded paper and allow for natural hiding, burrowing and nesting behavior.
- Animals that undergo surgical procedures will be given pain relief in form of Temgesic.
- A plan for monitoring the well being of the mice as well as humane endpoints are in place to make sure that any discomfort the animals might experience due to engraftment of tumor tissue/cells is kept to a minimum.
The mice applied for in this application will be used to generate new PDX models from patient tissue, re-establish models from human cell lines/cryoperserved tissue and to maintain the growth of existing xenograft models. The tissue generated will be used to support the department of Tumor biology ex vivo and in vivo activities.
2 Impact on animal welfare:
The animals used in this application will be subjected to various engraftment procedures and be handled/restrained on a weekly basis (check ups). The tumor tissue grafted into the mice will be allowed to grow to endpoint before it is passaged into new mice. Animals that are getting close to end point might experience weight loss and deteriorating health. Depending on the growth rate of the xenograft the life expectancy for the animals after engraftment will vary from 30-90 days.
3 Benefit:
Patient-derived xenografts (PDX) are models of cancer where the tissue or cells from a patient's tumor are implanted into an immune deficient or humanized mouse. PDX models simulate human tumor biology allowing for natural cancer progression, and offer the most translational research model for evaluating efficacy.
4 Number of animals:
In this application we plan to use up to 2400 Athymic nude mice over a period of four years.
5 The three Rs
Replacement:
- So far no alternatives has been discovered that can be a substitute for the complex functions of a living, breathing, whole-organ system with pulmonary and circulatory structures like those in humans. Until such a discovery, animals must continue to play a critical role in helping researchers understand the biology of cancer.
Reduction:
- The xenograft models that are not in use will be cryopreserved and stored untill needed.
- Passaging is done into a small number of animals, usually 2-3 animals per passage.
- Tissue generated for ex vivo experiments will be shared between researchers.
Refinement.
- Environmental enrichment will be provided in form of the addition of cardboard houses, plastic tubes, and/or shredded paper and allow for natural hiding, burrowing and nesting behavior.
- Animals that undergo surgical procedures will be given pain relief in form of Temgesic.
- A plan for monitoring the well being of the mice as well as humane endpoints are in place to make sure that any discomfort the animals might experience due to engraftment of tumor tissue/cells is kept to a minimum.