In ovo exposure and effects of two emerging compounds on birds
The experiment is conducted to study the effects of a regulated compound and its still unknown substitute (seperately and together) on the avian embryo. Perfluorooctanesulfonic acid (PFOS), a chlorinated polyfluorinated ether sulfonate (F-53B) and their mixture will be injected in ovo, using broiler chicken (Gallus gallus domesticus) as a model species for birds.
One hundred and fifty eggs will be injected and embryos will be decapitated right after hatching. Embryos will be measured and the liver will be sampled. Effects on development, metabolism and oxidative stress will be investigated.
The results from the study will generate data important for risk assessment of exposure to these compounds in birds. information about effects of PFOS on avian embryos is abundant but very diverging in its results and information on the effects of its replacing compound F-53B is still not available. The latter prevents the use of computer modellling and in vitro assays lack the possibilty to study whole organism responses and effects on development and pipping success. Due to our experience with similar experiments in the past we have been able to refine the methods of injection, incubation and euthanasia as much as possible. By the use of chicken eggs instead of quail eggs, we are able to diminish the time of potential distress, since chickens are bigger and can be sampled at an earlier stage in the development.
This is a study which is part of NewRaptor project (NFR, 230465/F20).
One hundred and fifty eggs will be injected and embryos will be decapitated right after hatching. Embryos will be measured and the liver will be sampled. Effects on development, metabolism and oxidative stress will be investigated.
The results from the study will generate data important for risk assessment of exposure to these compounds in birds. information about effects of PFOS on avian embryos is abundant but very diverging in its results and information on the effects of its replacing compound F-53B is still not available. The latter prevents the use of computer modellling and in vitro assays lack the possibilty to study whole organism responses and effects on development and pipping success. Due to our experience with similar experiments in the past we have been able to refine the methods of injection, incubation and euthanasia as much as possible. By the use of chicken eggs instead of quail eggs, we are able to diminish the time of potential distress, since chickens are bigger and can be sampled at an earlier stage in the development.
This is a study which is part of NewRaptor project (NFR, 230465/F20).